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Image Search Results
Journal: Microbial Genomics
Article Title: A step forward for Shiga toxin-producing Escherichia coli identification and characterization in raw milk using long-read metagenomics
doi: 10.1099/mgen.0.000911
Figure Lengend Snippet: Boxplot representing the minimal coverage required to assemble eae -positive STEC. The minimal coverage at which stx and eae were co-localized on the same contig is represented depending on the long-read assembler used on subsampled MinION data of 10 eae -positive STEC strains. The minimal coverage required for eae -positive STEC identification was confirmed when stx and eae genes were found on the same contig in all of the following assemblies with higher coverages (one exception was accepted). Flye was the most efficient to assemble eae -positive STEC with a genome coverage up to 35×, although the difference with Canu and Raven assemblers was not significant (Kruskal–Wallis test, P value >0.05).
Article Snippet: The resulting P values were represented on an
Techniques:
Journal: Microbial Genomics
Article Title: A step forward for Shiga toxin-producing Escherichia coli identification and characterization in raw milk using long-read metagenomics
doi: 10.1099/mgen.0.000911
Figure Lengend Snippet: E. coli reads proportion and E. coli assembly metrics obtained from artificially contaminated raw milk. Raw milk samples were artificially contaminated with STEC at the concentrations of 0.5×10 3 c.f.u. ml −1 ( n =3; 500), 0.5×10 2 c.f.u. ml −1 ( n =4, 50) or 0.5×10 1 c.f.u. ml −1 ( n =5, 5) of raw milk and enriched at 37 °C in the presence of acriflavine (37+A). (a) Boxplot representing the percentage of extracted E. coli reads. (b) Dotplot representing assembly metrics obtained from Flye assemblies on extracted E. coli reads. A triangle represents the identification of stx and eae genes on the same contig, whereas their identification on two different contigs is represented using a circle. Milk batches are represented using different colours.
Article Snippet: The resulting P values were represented on an
Techniques: